As noted above, behavioral testing in the present study was conducted in 3xTg-AD mice and WT controls at 7 months of age. To determine if alcohol intake altered motor function in this age range, we evaluated 3xTg-AD and WT mice in the open field and rotarod tests during the first week after alcohol exposure. In the open field test, 3xTg-AD mice showed levels of spontaneous locomotor activity and habituation that were not different than WT mice (Fig. 4D). There was also no effect of genotype or history of alcohol intake. Three-way repeated measure (RM)-ANOVA (genotype × alcohol × time) found a significant main effect of time [F(2.040, 32.64) = 34.84, P < 0.0001] with no main effect of genotype or alcohol intake, and no interactions among the variables. This indicates that mice from all groups showed similar levels of activity and habituation to the environment, which suggests the absence of deficits in gross motor function or habituation learning. When tested on the rotarod, performance of all mice improved as a function of repeated trials (Fig. 4E). Three-way ANOVA showed a
