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Chunk #6 — METHODS — Experiment 1: The Effect of D-Lys3-GHRP-6 on preference to alcohol in a two-bottle choice experiment

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Ghrelin receptor antagonism decreases alcohol consumption and activation of perioculomotor urocortin-containing neurons.
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saline; Tocris Bioscience, Ellisville, MO) and 30 min later were allowed access to water and 15 % ethanol for 4 h. Volumes of the fluids consumed were recorded and the mice euthanized by an overdose of CO2. All injections were intraperitoneal and administered 2 h and 30 min into the dark cycle and drinking tubes replaced 30 minutes later, at 12 pm.