saline; Tocris Bioscience, Ellisville, MO) and 30 min later were allowed access to water and 15 % ethanol for 4 h. Volumes of the fluids consumed were recorded and the mice euthanized by an overdose of CO2. All injections were intraperitoneal and administered 2 h and 30 min into the dark cycle and drinking tubes replaced 30 minutes later, at 12 pm.