On days 1 to 3, as in Experiment 1, the mice were habituated to saline (0.3 ml) injections as in Experiment 1. On days 4 to 6, mice received saline injections and 30 min later were given access to 20 % ethanol (v/v, made in tap water) for 2 h. On day 7, the mice were divided into groups matched for alcohol intakes and either injected with 0.3 ml saline or 400 nmol D-Lys3-GHRP-6. Thirty minutes later, they were allowed access to 20 % ethanol for 4 h. The volumes consumed were recorded at the end of the access periods and on day 7, at the conclusion of the 4 h, the mice were euthanized as above and brains and blood samples were collected. As before, all injections were intraperitoneal and carried out 2 h and 30 min into the dark cycle and drinking tubes replaced 30 minutes later, at 12 pm.
