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Chunk #8 — METHODS — Experiment 3: The Effect of D-Lys3-GHRP-6 on c-Fos induction by systemic ethanol

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Ghrelin receptor antagonism decreases alcohol consumption and activation of perioculomotor urocortin-containing neurons.
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Mice were habituated to saline injections for 4 days. On day 5, either 0.3 ml saline or 400 nmol D-Lys3-GHRP-6 was injected intraperitoneally. Thirty minutes later, either saline or 2.5 g/kg ethanol was injected, also intraperitoneally, and after 90 min the mice were euthanized as above and brains were collected for immunohistochemical processing.