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Chunk #9 — METHODS — Experiment 4: Blood ethanol concentrations after D-Lys3-GHRP-6 pre-treatment and systemic ethanol

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Ghrelin receptor antagonism decreases alcohol consumption and activation of perioculomotor urocortin-containing neurons.
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Mice were injected with either 0.3ml saline or 400 nmol D-Lys3-GHRP-6 which was followed 30 min later by 2.5 g/kg ethanol i.p. The mice were then euthanized at two time-points: 1 h or 2 h after the second injection. Blood samples were collected and blood ethanol concentrations (BECs) were determined.