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Chunk #10 — METHODS — Blood ethanol concentration (BEC) measurements

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Ghrelin receptor antagonism decreases alcohol consumption and activation of perioculomotor urocortin-containing neurons.
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Samples from both Experiments 2 and 4 were treated similarly, i.e. blood samples were centrifuged and plasma removed and frozen at −20°C until analyzed. Blood ethanol concentrations (BECs in mg/dl) were obtained using the GL5 Analyser (Analox Instruments USA, Lunenburg, MA).