1:2000), mouse anti-Tra-1–60 (Millipore Sigma MAB4360, 1:1000), and mouse anti-Gad-67 (Abcam ab26116, 1:500). Secondary antibodies used included goat anti-chicken IgY-Alexa Fluor 546 (Invitrogen A-11040, 1:500), goat anti-rabbit IgG-Alexa Fluor 488 (Invitrogen A-31556, 1:500) and goat anti-mouse IgG-Alexa Fluor 546 (Invitrogen A-11030, 1:500). Images were taken on a Zeiss LSM700 confocal microscope. All images presented are maximum intensity projections of 5 plane z-stacks obtained at a step size of 1 μm.
