Chunk #18 — Materials and methods — Immunocytochemistry and imaging analysis

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Differential sensitivity of human neurons carrying μ opioid receptor (MOR) N40D variants in response to ethanol.

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For synaptic quantification, 63X images were taken for iNs stained for MAP2 and Synapsin. The number of Synapsin+ puncta, as well as puncta size and fluorescence intensity of Synapsin staining, were identified using Intellicount (Fantuzzo, Miorabella, et al., 2017, a high-throughput, fully automated synapse quantification program.