Cytochrome P-450 (CYP2E1) is the key enzyme of the microsomal pathway of ethanol oxidation. It is significantly expressed in different brain cell compartments, including the endoplasmic reticulum, the plasma membrane, the Golgi apparatus, as well as mitochondria (Yu et al., 2021). Brain endogenous substrates of CYP2E1 are arachidonic acids, linoleic acids, oleic acids, gluconeogenic precursors and estrogenic metabolites (Ohe et al., 2000), and xenobiotic substances (Kuban and Daniel, 2021). In the brain, CYP2E1 is involved in the oxidation of ethanol and ROS production, which can result in increased lipid peroxidation (Zimatkin et al., 2006), increased permeability of the blood-brain barrier and neurodegeneration (Jin et al., 2013).
