the WT and the KI mice, but ethanol had no effect on PPARα levels in the KO mice (Fig. 7B). A downstream target of PPARα, peroxisomal acyl CoA oxidase, responded in a similar manner, being decreased by ethanol in WT and KI mice but no effect was found with KO mice (Fig. 7C). Previously [20], we found that levels of PPARα mRNA but not protein were decreased by ethanol in WT mice while levels of AOX mRNA and protein were decreased. In the current study, levels of AOX as well as PPARα protein were decreased by ethanol in the WT mice. The only difference between the two studies was the time of ethanol feeding, three (current study) versus four weeks [20] so it is not clear why the effect of ethanol on PPARα protein but not AOX protein levels differ between the 2 studies.
