Chunk #4 — 2. Materials — 2.1. Reagents — 2.1.2. Growth media and supplements

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Differentiation of human pluripotent stem cells into Medial Ganglionic Eminence vs. Caudal Ganglionic Eminence cells.

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DMEM (Life Technologies, Carlsbad, CA, cat. no. 11965–118)Essential 8 medium kit (E8 medium; Life Technologies, Carlsbad, CA, cat. no. A1517001)ROCK inhibitor Y-27632 (Selleck Chemicals, Houston, TX, cat. no. S1049)Knockout serum replacement (KSR; Life Technologies, Carlsbad, CA, cat. no. 10828–028)Penicillin-streptomycin (Life Technologies, Carlsbad, CA, cat. no. 15140–163)2-Mercaptoethanol (Sigma-Aldrich, St. Louis, MO, cat. no. M3148)l-Glutamine, 200 mM (Life Technologies, Carlsbad, CA, cat. no. 25030–164)LDN193189 (Stemgent, Cambridge, MA, cat. no. 04–0074)SB431542 (Selleck Chemicals, Houston, TX, cat. no. S1067)IWP-2 (Selleck Chemicals, Houston, TX, cat. no. S7085)SAG (Cayman Chemical, Ann Arbor, MI, 11914)Recombinant human FGF-8 (PeproTech, Rocky Hill, NJ, cat. no. 100–25)Recombinant human FGF-19 (PeproTech, Rocky Hill, NJ, cat. no. 100–32)N-2 supplement (Life Technologies, Carlsbad, CA, cat. no. 17502–048)DMEM-F12 (Life Technologies, Carlsbad, CA, cat. no. 11320– 082)Recombinant human GDNF (PeproTech, Rocky Hill, NJ, cat. no. 450–10)Recombinant human BDNF (PeproTech, Rocky Hill, NJ, cat. no. 450–02)HBSS, calcium, magnesium, no phenol red (Life Technologies, Carlsbad, CA, cat. no. 14025–134)Boc-Asp(OMe) fluoromethyl ketone (BAF; Sigma-Aldrich, St. Louis, MO, cat. no. B2682)