Chunk #5 — 2. Materials — 2.1. Reagents — 2.1.3. Enzymes and other reagents

Source

Differentiation of human pluripotent stem cells into Medial Ganglionic Eminence vs. Caudal Ganglionic Eminence cells.

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Matrigel, hESC-qualified matrix (BD Biosciences, San Jose, CA, cat. no. 354277)DPBS, without calcium and magnesium (Life Technologies, Carlsbad, CA, cat. no. 14190–250)EDTA (Sigma-Aldrich, St. Louis, MO, cat. no. E6758)Poly-l-ornithine solution (PLO; Sigma-Aldrich, St. Louis, MO, cat. no. P4957)Fibronectin (FN; Sigma-Aldrich, St. Louis, MO, cat. no. F0895)Trypsin-EDTA solution, 0.5% (Life Technologies, Carlsbad, CA, cat. no. 15400-054)Fixation/permeabilization solution kit (BD Biosciences, San Jose, CA, cat. no. 554714)Bovine serum albumin (BSA; Sigma-Aldrich, St. Louis, MO, cat. no. A9418)Saponin (Sigma-Aldrich, St. Louis, MO, cat. no. 47036)TRIzol reagent (Life Technologies, Carlsbad, CA, cat. no. 15596-026)PureLink RNA mini kit (Life Technologies, Carlsbad, CA, cat. no. 12183018A)SuperScript II reverse transcriptase (Life Technologies, Carlsbad, CA, cat. no. 18064-014)Oligo d(T)12–18 (Gene Link, Hawthorne, NY, cat. no. 26–4000-05)SYBR green I nucleic acid gel stain (Sigma-Aldrich, St. Louis, MO, cat. no. S9430)Formaldehyde, 37% (Electron Microscopy Sciences, Hatfield, PA, cat. no. 15686)Normal donkey serum (NDS; Sigma-Aldrich, St. Louis, MO, cat. no. D9663)Triton X-100 (Sigma-Aldrich, St. Louis, MO, cat. no. X100)Hoechst 33342 (Life Technologies, Carlsbad, CA, cat. no. H3570)Fluoromount-G (SouthernBiotech, Birmingham, AL, cat. no. 0100-01)Isoflurane (Sigma-Aldrich, St. Louis, MO, cat. no. 792632)Buprenorphine hydrochloride