Chunk #6 — 2. Materials — 2.1. Reagents — 2.1.3. Enzymes and other reagents

Source

Differentiation of human pluripotent stem cells into Medial Ganglionic Eminence vs. Caudal Ganglionic Eminence cells.

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donkey serum (NDS; Sigma-Aldrich, St. Louis, MO, cat. no. D9663)Triton X-100 (Sigma-Aldrich, St. Louis, MO, cat. no. X100)Hoechst 33342 (Life Technologies, Carlsbad, CA, cat. no. H3570)Fluoromount-G (SouthernBiotech, Birmingham, AL, cat. no. 0100-01)Isoflurane (Sigma-Aldrich, St. Louis, MO, cat. no. 792632)Buprenorphine hydrochloride (Sigma-Aldrich, St. Louis, MO, cat. no. B9275)Triple antibiotic ointment (Fisher Scientific, Pittsburgh, PA, cat. no. 19–075–383)Pentobarbital (Sigma-Aldrich, St. Louis, MO, cat. no. P0500000)Heparin sodium (Sigma-Aldrich, St. Louis, MO, cat. no. 1304005)Sodium chloride standard, aqueous solution (Fisher Scientific, Pittsburgh, PA, cat. no. 7213095)Sucrose (Fisher Scientific, Pittsburgh, PA, cat. no. S6–12)