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Chunk #18 — METHODS — EXPERIMENTAL ANIMALS: PHYSIOLOGIC AND BEHAVIOR ASSESSMENTS — Alcohol Consumption

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CRH haplotype as a factor influencing cerebrospinal fluid levels of corticotropin-releasing hormone, hypothalamic-pituitary-adrenal axis activity, temperament, and alcohol consumption in rhesus macaques.
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Young adult macaques (age 3.5–5, N=74) were allowed to freely consume an aspartame-sweetened 8.4% (v/v) alcohol solution for one hour per day, 5 days a week in their home run. This method consisted of three phases, which have previously been reported 17. (1) Spout Training; (2) Initial Alcohol Exposure; and (3) Experimental Period. During the 6-week experimental phase, alcohol and vehicle were dispensed 5 days a week (Monday-Friday) from 1300 to 1400 while the animals were in their home-cage environment. Animals were fitted with a collar implanted with an identifier chip so that volumes consumed could be recorded for each individual.