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Chunk #17 — METHODS — EXPERIMENTAL ANIMALS: PHYSIOLOGIC AND BEHAVIOR ASSESSMENTS — Behavior Assessments: Early Infant Homecage Behavior and Intruder Challenge Test

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CRH haplotype as a factor influencing cerebrospinal fluid levels of corticotropin-releasing hormone, hypothalamic-pituitary-adrenal axis activity, temperament, and alcohol consumption in rhesus macaques.
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randomly grouped animals were locked into the outdoor portion of their home run, an enclosure measuring 2.64 m wide × 3 m long × 2.44 m high. After ten minutes, the intruder animal’s cage was placed directly at the front of the enclosure and behavioral scoring of the test subjects was initiated. One observer was assigned to each test subject, which was observed for 30 minutes using focal animal continuous recording. Behaviors recorded are listed in Table S1. Behaviors were generally scored in seconds in duration. Vocalization, aggression, and approach intruder were scored in frequency. Interobserver reliability was established at greater than r = 0.85 for all behaviors.