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Chunk #40 — Methods — Network morphology

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Sustained synchronized neuronal network activity in a human astrocyte co-culture system.
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To measure neurite outgrowth (MAP2), a multi-tier approach was used, which was based on a dedicated in-house developed analysis for dense neuronal networks, named MorphoNeuroNet59. The procedure selectively segments high and low-intensity features in the image and combines them into a single mask.