Chunk #41 — Methods — Network morphology

Source

Sustained synchronized neuronal network activity in a human astrocyte co-culture system.

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Text

Synaptic puncta (vGLUT1, GAD65) were pre-processed by means of a rolling ball background subtraction, Laplace filtering, automated thresholding1760, particle size filtering and counting of the puncta. Density of puncta was expressed as the number of positive puncta per mm2 MAP2-positive surface.