The in silico PCR tool lets users submit a pair of PCR primers and returns the target sequence that those primers will produce. The initial version of this tool worked only on genomic sequence, but has since been extended on the human and mouse browsers to allow the query of transcribed sequences as well. When ‘UCSC Genes’ is selected as the target, primers that occur within the exonic regions of a gene, even those spanning long introns, will produce PCR product sequence for all gene isoforms with the introns removed. Clicking the links on the results page displays graphical representations of the PCR products in the browser, with the location of the primers indicated.
