Interestingly, 27 of the 112 shared DEGs (24%) showed a discordant direction of effect in at least one of the four brain regions. This correlated well with the pairwise correlation of shared DEGs that shared the direction of effect (70% to 82%; Fig. 2d). However, this proportion of sharing dropped substantially when effect size was considered (0.22–0.44; Fig. 2d). Correspondingly, we found a large number of brain region-specific DEGs (1,360 (52.9%); Fig. 2e), which increased with isoform-level analysis (transcript (63.6%), exon (67.6%) and junction (69.7%)). This aligns with other studies showing isoform-level brain region specificity27.
