The effect of alcohol on splicing in the brain has not been well studied. We employed MATS program18 which detects such mis-splicing events as 5′ alternative splice sites (5′-SS), 3′ alternative splice sites (3′-SS), intron retention (IR), exon skipping (ES), and mutually exclusive exons (MEE). In all four brain regions, these mis-splicing events were detected, with ES being the most common event and imbalance in MEEs—the second most common event (Fig. 2). Mis-splicing in AUD was much more profound than a differential expression: 1421 mis-splicing events were detected in SFC, 394 in NA, 1317 in BLA, and 469 in CNA. Frequency of skipped exons varied from 237 in NA to 779 in SFC. The frequency of imbalanced MEEs varied from 122 in CNA to 486 in BLA. The frequency of 5′-SS, 3′-SS, and IR was <100 in all four brain regions.
