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Chunk #10 — Introduction — Social Interaction

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Alcohol during adolescence selectively alters immediate and long-term behavior and neurochemistry.
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from PND 31–34. Specifically, in the morning, all rats underwent social isolation in a holding cage and water administration to the demonstrator after the initial 30 minutes of social isolation followed by an additional 30 minutes of social isolation. Rats were then given the opportunity to socially interact with an alcohol-free peer in the initially most-preferred chamber for 30 minutes. Four hours later, the same paradigm was repeated, however the same demonstrator was orally administered ethanol (0.5 or 1.5 g/kg) or water in control rats and the dyad were confined to the alternate chamber for 30 minutes. Each adolescent observer socially interacted with the same adolescent demonstrator for all social interaction sessions. However, the demonstrator was sober in the morning sessions of social interaction and intoxicated in the afternoon sessions of social interaction. Control rats socially interacted with the same sober peer for all social interaction sessions. For each dose tested in which the observer socially interacted with an alcohol-intoxicated or non-intoxicated demonstrator, separate rats were used, for a total of three groups of observer-demonstrator dyads (control, 0.5 and 1.5 g/kg). This cycle was repeated three additional times for a total of four pairings with an alcohol-free peer and four