For whole blood and plasma measurements, mouse blood was collected from the tail vein into EDTA-coated capillary tubes (StatSpin). Blood glucose and triglyceride levels were measured with a OneTouch Ultra glucometer (LifeScan) and a CardioChek PA analyzer, respectively. Plasma concentrations of insulin (Mercodia) and leptin (Alpco) were measured by standard immunoassay methods according to the manufacturer’s protocol. For serum measurements, blood was collected by cardiac puncture from anesthetized (350 mg/kg chloral hydrate, i.p.) mice. Serum levels of luteinizing hormone (LH) and follicle-stimulating hormone were determined at the University of Virginia Center for Research in Reproduction Ligand Assay and Analysis Core.
