Mice were individually housed for at least 3 days prior to the measurement of food intake or calorimetry. Daily food intake of ad libitum mice was determined by measuring the weight of food pellets on consecutive days. Locomotor activity, oxygen consumption and carbon dioxide production were simultaneously measured with a Comprehensive Lab Animal Monitoring System (Columbus), as previously described 44. For nocturnal food intake studies, food was removed from individually housed mice 1h prior to the onset of the dark cycle. Saline, leptin (2μg/g), and MTII (2μg/g) were administered by intraperitoneal injection 30 min prior to the onset of the dark cycle. Food was replaced at the onset of the dark cycle and food intake was measured after 90 min.
