Lentivirus was packaged in 293T HEK cells grown in 293T media (IMEM (Invitrogen), 10% FBS (Gemini), 1xGlutamax(Invitrogen)). 293T cells were transfected with Polyethylenimine (PEI) (Polysciences). Per 15-cm plate, the following solution was prepared, incubated for 5 minutes at room temperature and added drop-wise to plates: 12.2 µg lentiviral DNA, 8.1 µg MDL-gagpol, 3.1 µg Rev-RSV, 4.1 µg CMV-VSVG, 500 µl of IMDM and 110 µl PEI (1 µg/µl) and vortexed lightly. Medium was changed after three hours and the virus was harvested at 48 and 72 hours post transfection.
