Caco-2 colon cancer cells grown in 96-well plates were transfected with 100ng/well of Snail reporter plasmid (gift of A.G. de Herreros)(Barbera et al., 2004) or CDH1::LUC E-cadherin reporter (gift of L. Larue)(Julien et al., 2007). Values were read 8h after initial stimulation with 0.2% (43 mM) alcohol for the Snail reporter and after 24h and 48h for the E-cadherin reporter. Cells were co-transfected with 1ng SV40 renilla luciferase plasmid (Promega, Madison, WI) and developed using the Stop and Glow kit (Promega), read on a Lumioskan Ascent plate reader (Thermo Labsystems, Waltham, MA), and data normalized to renilla values to control for transfection efficiency.
