right hemisphere and pulverized in liquid nitrogen. The tissue was shipped in tubes appropriate for DNA or RNA extraction to ISMMS as homogenized tissue in trizol for RNA extraction and dry pulverized tissue for DNA extraction.Pitt: At autopsy, the right hemisphere of each brain was blocked coronally, immediately frozen and stored at −80 °C. Samples for the study contained only the gray matter of DLPFC where Brodmann area 9 from the right hemisphere was cut on a cryostat and collected in tubes appropriate for DNA or RNA extraction. The DNA and RNA tubes were shipped on dry ice to ISMMS as homogenized tissue in trizol for RNA extraction and thinly sliced tissue for DNA extraction. Specimens from Pitt were provided as matched case/control pairs. These were perfectly matched for sex, and as closely as possible for age and race. Members of a pair were always processed together.
