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Chunk #19 — Materials and Methods — Mammalian model organism studies — Mouse studies

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Genomewide Association Study of Alcohol Dependence Identifies Risk Loci Altering Ethanol-Response Behaviors in Model Organisms.
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We queried selected candidate genes for localization to ethanol behavioral QTL intervals using the Mouse Genome Informatics (MGI) tool set. We queried published expression microarray and ethanol behavioral response datasets within the curated GeneNetwork web-based resource of genetic, phenotypic and genomic data for evidence that basal candidate gene expression correlated with measured ethanol behavioral phenotypes in C57BL/6J × DBA/2J recombinant inbred (BXD) mouse lines. All expression data were from Affymetrix M430 microarrays (Affymetrix, Santa Clara, CA). We included saline or air treated BXD control whole brain (GN113), nucleus accumbens (NAc) (GN156), prefrontal cortex (PFC) (GN135), and ventral tegmental area (VTA) (GN228) (Wolen et al., 2012).