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Chunk #6 — Materials and Methods — Study subjects

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Cis-regulatory variants affect CHRNA5 mRNA expression in populations of African and European ancestry.
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who were selected from the COGA dataset based on the genotypes of rs3841324 from our previous study [20]. In the African American subset, 24 subjects are homozygous for the major allele of rs3841324, 21 subjects are homozygous for the minor allele, and 15 subjects are heterozygous. In the European American subset, 21 subjects are homozygous for the major allele of rs3841324, 18 subjects are heterozygous, and 21 subjects are homozygous for the minor allele. The Washington University IRB reviewed the protocol using brain tissue and determined it was exempt from approval. The study using the COGA dataset was approved by IRB at all participating institutions. All data were analyzed anonymously.