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Chunk #13 — Methods — Animal Studies — Gene expression profiling in alcohol-preferring rat strains

Source
Genome-wide association study of alcohol dependence.
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yes

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Preparation of brain samples and RNA isolation are described in the Supplemental Text. Target preparation was done for individual samples from caudate putamen and amygdala using 5 μg of total RNA. Hybridization to RG U34A arrays, staining, washing and scanning of the chips were performed according to the manufacturer′s technical manual (Affymetrix, Santa Clara, CA).