V6.5 ESCs were infected with shRNAs targeting lincRNAs, protein-coding genes, and 21 negative controls. After 8-days, RNA was extracted and mRNA levels of the Oct4, Nanog, Sox2, Klf4, and Zfp42 pluripotency markers were analysed using qPCR. Primer sequences are listed in Supplemental Table 9. Each sample was normalized to Gapdh levels. Significance was assessed compared to the negative control hairpins using a one-tailed t-test.
