After a 7 day infection, Nanog-Luciferase cell viability was measured using AlamarBlue (Invitrogen; DAL1025). AlamarBlue was mixed with mES media in a 1:10 ratio, added to the cells and incubated at 37°C for 1 hour. Absorbance readings at 570nm were taken. To control for possible effects due to virus titer, we measured AlamarBlue incorporation on both puromycin treated and non-puromycin treated samples for each infection.
