Chunk #59 — Materials and methods — Mutation detection procedure — Step 4: detection in the tested sample — Filter 6

Source

Detection of low prevalence somatic mutations in solid tumors with ultra-deep targeted sequencing.

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Using the error rate generated from the calibration sample, we calculated the binomial test P-values and ranked the candidate position in the tested sample accordingly. We kept all positions above the P-value determined by the MCC threshold obtained in the calibration sample.