Chunk #58 — Materials and methods — Mutation detection procedure — Step 4: detection in the tested sample — Filter 5

Source

Detection of low prevalence somatic mutations in solid tumors with ultra-deep targeted sequencing.

Embedded

yes

Text

In order to prevent bias due to coverage discrepancies between the calibration and the tested sample, we rejected all bases of the tested sample with a coverage depth lower than the 5th coverage percentile across all bases of the calibration sample.