Antibiotic-selected dCas9-effector NPCs per well (4.0 × 105) were added to growth factor-reduced Matrigel-coated 24-well plates in NPC medium containing 1 μg/mL puromycin. SNAP91 gRNA#2 IVT product (600 ng) and 2 μL of EditPro Stem Transfection Reagent (MTI-GlobalStem, no. GST-2174), diluted in 50 μL of Opti-MEM (Thermo Fisher Scientific, no. 31985062) was added drop wise directly after. Cells were harvested 48 hr later.
