Total RNA was extracted using TRIzol following the manufactures instructions. Transcript analysis was carried out using a QuantStudio 7 Flex Real-Time PCR System using the Power SYBR Green RNA-to-Ct Real-Time qPCR Kit for primers (all Thermo Fisher Scientific). RNA template (50 ng) was added to the PCR mix, containing primers detailed in Table S3 (Thermo Fisher Scientific). qPCR conditions were as follows, 48°C for 15 min, 95°C for 10 min followed by 40 cycles (95°C for 15 s, 60°C for 60 s).
