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Chunk #8 — 2. Materials and Methods — 2.1 Animal Binge drinking protocol

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Gene expression changes in serotonin, GABA-A receptors, neuropeptides and ion channels in the dorsal raphe nucleus of adolescent alcohol-preferring (P) rats following binge-like alcohol drinking.
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Peri-adolescent male P (alcohol preferring) rats were maintained on a reverse light-dark cycle and given free-choice access to ethanol using a multiple-scheduled-access procedure, as described in Bell et al. (2011). There were 10 animals in the water control group and 11 in the ethanol binge-drinking group. Animals were given ad libitum access to food and water and those in the drinking group were given access to ethanol (15 and 30% ethanol solutions concurrently) in 3 x 1-h sessions per day for 5 consecutive days/week, starting at 28 days of age. The three 1hr ethanol access sessions were scheduled so that the first 1hr session started at dark onset (1000 h), the second 1 h session began 2 h after the end of the first session (1300 h) and the third/last 1hr session began 2 h after the end of the second session (1600 h). The animals used in this study are the same ones described in McBride et al. (2014). A graph of the 15-day ethanol drinking data is given in McBride et al. (2014). In general, during week 1,