As inhibition of 2-AG hydrolysis with MAFP has been shown to increase DSI duration in the hippocampus (Hashimotodani et al, 2007b), we determined the effects of 1 µM MAFP on DSI in the BLA. Bath application of MAFP for >30 min before recording significantly prolonged DSI (Figure 6a). Two-way ANOVA showed a significant effect of MAFP (p < 0.001), time (p < 0.001), and a significant interaction (p < 0.05). Summary data indicate that MAFP did not significantly increase maximal DSI compared with vehicle (vehicle 80.1 ± 5.1% N = 12 vs MAFP 66.3 ± 5.4% N = 8, p > 0.05), but robustly increased the late component of DSI (vehicle 102.5 ± 4.4% N = 12 vs MAFP 74.7 ± 3.6% N = 8, p < 0.001). These data indicate that DSI duration is limited by eCB degradation.
