IPA identified the CI treatment as containing the most statistically significant networks and biological functions in PFC (Table S3). Neurological disease and genetic disorders were the top identified biological functions and were also represented among the functions of the top five networks, thus we chose to investigate these annotation groups further. Since IPA functional groups do not represent a known biological network per se, we used the top biological function results to derive our own network. Briefly, the two genetic disorder-related annotation groups containing the largest numbers of molecules (“genetic disorder” and “Huntington’s disease”) were combined and their molecules connected by knowledge base relationships to form a preliminary network. This network was “grown” once, adding a limited number of related molecules with high specific connectivity to the network. The resulting PFC network contains a variety of genes involved with dopaminergic signaling, as well as genes related to immune function (Figure 4A). Notably, when data from each of the other studies are overlaid on this CI dopaminergic network, similarities between LPS and CI treatments are revealed. These similarly regulated genes are identified in the tables to the left in Figure 3.
