The second step of ethanol metabolism is the oxidation of acetaldehyde to acetate, catalyzed by aldehyde dehydrogenase (ALDH). We tested the effects of knocking down function of each of 10 C. elegans ALDH-like enzymes using RNAi. Inactivation of either alh-6 or alh-13 caused ethanol hypersensitivity (Figure 4a).
