We wished to determine if genes that show alterations in transcript levels following ethanol exposure also contribute to ethanol behavioral responses. To accomplish this, we assayed 107 strains that harbored transposon insertion lesions in 61 ethanol-regulated genes for locomotor activity and sedation sensitivity in response to ethanol vapor in the rapid tolerance paradigm (Table S5). We chose genes for mutant analysis based on one or more of the following criteria: (i) highly significant gene expression regulation by exposure to ethanol vapor but little to no regulation by exposure to humidified air, (ii) onset of gene expression regulation within 90 minutes of termination of ethanol vapor exposure to bias toward genes that may contribute to tolerance, (iii) sequence similarity to mammalian gene products, (iv) previous data implicating the gene or related genes in cellular processes in the central nervous system, and (v) availability of transposon insertional lesions or other genetic tools that allowed for rapid behavioral testing. To minimize the influence of genetic background, all strains were backcrossed to the Berlin parental strain prior to behavioral testing. The Berlin strain was
