Chunk #32 — Results — The reproducibility of array data using QuantiGene, a PCR-independent platform to confirm expression results

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Quality control parameters on a large dataset of regionally dissected human control brains for whole genome expression studies.

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We validated the exon array results in a subset of 12 individuals by comparing the normalised mRNA expression level for three genes (LRRK2, SCN8A and MAPT), in four brain regions (CRBL, OCTX, PUTM and WHMT). We observed a similar regional pattern of expression across the two platforms for both high (SCN8A and MAPT) (Fig. 5) and low expression transcripts in brain (LRRK2) (Fig. 6).