Chunk #51 — STAR METHODS — METHOD DETAILS — Recombinant ApoE and RAP proteins, and glia-secreted factors

Source

ApoE2, ApoE3, and ApoE4 Differentially Stimulate APP Transcription and Aβ Secretion.

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For production of recombinant RAP, pGEX-KG-RAP (generous gift of Dr. Joachim Herz, UT Southwestern Medical Center) was expressed in BL21 bacteria, and GST-RAP was purified as described above, except that thrombin cleavage (10 U per mg protein, overnight at 4°C) was used instead of rhinovirus 3C protease as a final step.