Chunk #8 — Methods — Analysis of Locomotion (crawling)

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Ethanol metabolism and osmolarity modify behavioral responses to ethanol in C. elegans.

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The effects of osmolarity on crawling were assessed as above, except that assay plates were either made of standard NGM or of Dent's Saline Solution (10 mM D-glucose, 10 mM HEPES, 140 mM NaCl, 6 mM KCl, 3 mM CaCl2, 1 mM MgCl2, pH 7.4) with 2% agar.