Time course analysis was performed as above except that single animals were placed on plates and video images were recorded every 0.5 seconds for 15 minutes. The speed for each interval was averaged for 6 worms. The speeds were binned for each 1-minute period and an average was calculated. A one-way ANOVA was performed with a significance value of P < 0.01 using Prism v5.0 (GraphPad Software), with Bonferroni post-hoc tests comparing all bins.
