Our study used TRIzol reagent (Life Technologies, USA) to extract total RNA from cells and tissues. A NanoDrop spectrophotometer (Thermo Fisher Scientific, USA) was used to determine the concentration and quality of extracted RNA with absorbance at 260/280 nm. We utilized a One-Step SYBR PrimeScript RT-PCR kit (Takara Bio, Japan) to measure the relative expression levels of TAF15, LINC00665, STAU1, MTF1, YY2, and GTSE1, as well as the expression of GAPDH as an endogenous control, with an Applied Biosystems 7500 Fast RT-PCR system. Fold change was calculated in the relative quantification (2−ΔΔCt) manner.
