Chunk #19 — Results — Differentially Methylated Regions in the NAc Are Associated With Cross-Generational Effects of Adolescent THC Exposure in Adult F1 Offspring
Overall, the methylation changes in the THC group were modest within most DMRs, with a bias toward hypomethylated changes (Figure 1b). The mean absolute change in methylation was ~3.6%, and the largest difference observed was ~11.3%. A list of the top 30 DMRs according to methylation differences are shown in Table 1. Despite the overall modest differences observed, on a per locus basis, neighboring CpGs within DMRs exhibited concordant changes in DNA methylation between groups (Figure 2). Each DMR shown in Figure 3 contains at least three significant CpGs (q<0.01), with absolute mean methylation differences ranging between 2.5% and 11.3%.
