Chunk #18 — Results — Differentially Methylated Regions in the NAc Are Associated With Cross-Generational Effects of Adolescent THC Exposure in Adult F1 Offspring
Using this method, we identified 406 hypermethylated and 621 hypomethylated DMRs in offspring with parental THC exposure, including 5611 CpGs, 3758 (66.9%) of which were independently significant by logistic regression (q<0.01; Supplementary Table S1). The distribution of population-wide methylation levels at CpGs in our data set was bimodal, and the majority of CpGs had low levels of methylation, <20% (Figure 1a). However, CpGs within DMRs were on average more highly methylated compared with the background set of CpGs, especially CpGs within hypermethylated DMRs (Figure 1a). DMR size ranged from 15 to 824 bp (mean=191.29; Supplementary Figure S5a), and the number of CpGs per DMR ranged from 3 to 37 (mean=5.46), with an average CpG density of 0.046 (Supplementary Figure S5b). Importantly, read depths for CpGs within DMRs (mean=231.28; min=61.87; max=552.40) were similar to those observed genome-wide, indicating that random error resulting from reduced read depth did not underlie the methylation differences observed at these sites.
