Chunk #17 — Results — Differentially Methylated Regions in the NAc Are Associated With Cross-Generational Effects of Adolescent THC Exposure in Adult F1 Offspring
Differential methylation at the remaining 567 306 CpGs was first assessed using logistic regression. Although instances of methylation differences at single CpGs influencing changes in gene expression have been demonstrated, from a statistical perspective, given the burden of multiple testing, it can be difficult to assess the significance and reliability of variation observed at CpGs on a per site basis. To circumvent such issues, much recent work has focused on the development of methods to assess concurrent changes in methylation at neighboring CpGs (Bock, 2010). Furthermore, it has been shown that methylation levels are strongly correlated at CpGs that are closer in genomic space (Huynh et al, 2014). Thus, in this study we used a sliding-window approach (Supplementary Figure S2) to characterize a robust set of DMRs from our data set containing multiple statistically significant CpGs.
