Chunk #71 — Methods (full – for online materials) — Purification of blood cell types

Source

An atlas of active enhancers across human cell types and tissues.

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Dickinson, cat no 345763) and CD8 APC (Becton Dickinson, cat no. 345775) and sorted for CD3+CD8+ T cells. CD19+ and CD56+ cells were enriched from the CD8− fraction using magnetically labeled human CD19 and CD56 MicroBeads (Miltenyi). Enriched cells were stained with CD3 FITC (Becton Dickinson,cat no. 345763), CD19 PE (Becton Dickinson, cat no. 345777) and CD56 APC (Becton Dickinson, cat no.341027) and sorted into CD3+CD19+ B cells and CD3+CD56+ NK cells. Purification of blood monocytes is described elsewhere52.