eRING studies (Figure S1) were performed as previously described (Bhandari et al., 2009) using vapor from 33 or 50% ethanol. Flies have a strong, innate negative geotaxis response (vertical climbing in response to being startled). In eRING studies (Figure S1), bang-induced climbing distance in the continuous presence of ethanol vapor is measured at 1 min intervals. Flies become progressively sedated and T50 values (time to ethanol-induced 50% reductions in climbing) are determined as previously described (Bhandari et al., 2009).
